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KMID : 0043320150380040446
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Archives of Pharmacal Research
2015 Volume.38 No. 4 p.446 ~ p.455
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In vitro evaluation of the antioxidant and cytotoxic activities of constituents of the mangrove Lumnitzera racemosa Willd.
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Thao Nguyen Phuong
Luyen Bui Thi Thuy
Diep Chau Ngoc
Tai Bui Huu
Kim Eun-Ji
Kang Hee-Kyoung
Lee Sang-Hyun
Jang Hae-Dong
Cuong Nguyen The
Thanh Nguyen Van
Cuong Nguyen Xuan
Nam Nguyen Hoai
Minh Chau Van
Kim Young-Ho
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Abstract
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This study performed phytochemical and bioactive assessments of the mangrove Lumnitzera racemosa Willd. leaves. Bioassay-guided fractionation of the methanolic extracts led to the identification of thirty-six compounds (1?36), their structures were elucidated using detailed NMR spectroscopic and MS analysis. The extracts, fractions, and the isolated compounds were screened for potential antioxidant and cytotoxic activities. Antioxidant assays were performed using peroxyl radical-scavenging and reducing assays, whereas cytotoxicity was measured using MTT assays in HL-60 and Hel-299 cell lines. The methanolic extract, CH2Cl2 and n-BuOH fractions (10.0 μg/mL) exhibited potent antioxidant activity, with Trolox equivalent (TE) values of 24.94 ± 0.59, 28.34 ± 0.20, and 27.09 ± 0.37 (μM), respectively. In addition, the isolated compounds exerted cytotoxic effects in a dose-dependent manner; compounds 1 and 14 exhibited the most potent cytotoxicity in HL-60 cells, with IC50 values of 0.15 ± 0.29 and 0.60 ± 0.16 μM, respectively. To clarify the mechanism(s) behind these cytotoxic effects, we measured the time-dependent changes in apoptotic markers including the condensation and fragmentation of nuclear chromatin, and the downregulation of p-ERK1/2, p-AKT, and c-Myc levels.
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KEYWORD
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Lumnitzera racemosa, Combretaceae, Antioxidant, Cytotoxic, Apoptosis
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